產(chǎn)品詳情
簡單介紹：
重組鼠神經(jīng)生成素蛋白與其它公司提供的重組蛋白不同，rMuNeuropoietin蛋白產(chǎn)品為采用CFS的無細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來的重組蛋白，可表達(dá)出對細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組鼠神經(jīng)生成素蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組鼠神經(jīng)生成素蛋白
| Synonyms | Ctf2 |
| Species | Murine |
| Accession | P83714 |
| GeneID | 244218 |
| Unigene | Mm.249829. |
| Source | Escherichia coli. |
| Molecular Weight | 重組鼠神經(jīng)生成素蛋白Approximately 19.7 kDa, a single non-glycosylated polypeptide chain containing 182 amino acids. |
| AA Sequence | APISPSEPIG QAYSLALYMQ KNTSALLQTY LQHQGSPFSD PGFSAPELQL STLPSAAVSF KTWHAMEDAE RLSRAQGAFL ALTQHLQLVG DDQSYLNPGS PILLAQLGAA RLRAQGLLGN MAAIMTALGL PIPPEEDTLG FVPFGASAFE RKCRGYIVTR EYGHWTDRAV RDLALLKAKY SA |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組鼠神經(jīng)生成素蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 200 ng/ml, corresponding to a specific activity of > 5000 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, pH8.0, 500 mM NaCl, with 0.5 mM DTT. |
| Endotoxin | Less than 1 EU/μg of rMuNeuropoietin as determined by LAL method. |
| Reconstitution | 重組鼠神經(jīng)生成素蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組鼠神經(jīng)生成素蛋白 | |
| Background | Neuropoietin (NP; also known as cardiotrophin-2) is a 19.7 kDa member of the IL-6 family of cytokines. Considered to be the product of a gene duplication event involving cardiotrophin-1 (CT-1), it helps to define a subfamily within the IL-6 family that includes CT-1, CLC and CTNF. Mouse neuropoietin is synthesized as a 204 amino acid (aa) precursor that contains a 22 aa signal sequence and a 182 aa mature segment. The secreted molecule is characterized by the presence of four alpha-helices, typical of hematopoietic superfamily molecules. Mature murine neuropoietin shares 88%, 90% and 95% aa identity to chimpanzee, canine and rat neuropoietin, respectively. |
